Abstract:
Statement of problem:. The increased awareness of the dangers of cross-contamination with
hepatitis virus (HBV), (HCV) and HIV during dental procedures is having a growing impact on
attitudes toward infection control in the dental clinic and laboratories. .
Purpose of the study: The aim of this study was to evaluate the micro-organisms trans-mission
from the metal substructure to the dies after try-in stage..
Material and Methods: 10 cases have been selected and diagnosed. . Two final impressions
were taken for every case . The first impression was swabbed for microbiological testing and
discarded. The second impression was used for constructing the crown in aseptic conditions. The metal
substructure was triad in patient’s mouth . The metal substructure was placed on the die without using
any disinfecting material for . The die was swabbed and tested for the microorganisms counting .
Results: The count of Streptoococcus (α Hemoltica ) was the same results which obtained from the
contaminated impression.
Conclusion: The infection control measurements and guidelines should be strongly encouraged.
to ensure aseptic practice in dental clinics and laboratories to avoid cross contamination
