Antipseudomonal, Antioxidant, Anticoagulant, and Cytotoxic Activities of Novel Synthesized Heterocyclic Molecules

Abstract

In this work, we evaluate the antipseudomonal, antioxidant, anticoagulant, and cytotoxic activities of new spirooxindolopyrrolizidine-linked 1,2,3-triazole were investigated for their antipseudomonal, antioxidant, anticoagulant and cytotoxic activities. The antipseudomonal activity of these novel molecules was tested by the microdilution method. DPPH, ABTS, and β-Carotene tests were used to estimate the antioxidant properties. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests were measured to evaluate the anticoagulant potential and cytotoxic activity was assessed by MTT test. This study revealed that compounds P3 and P7 displayed excellent antipseudomonal activity against all tested P. aeruginosa Imp/R. with MIC values of 15.62μg/ml. the antioxidant activity demonstrated that all tested heterocyclic compounds exhibited important antioxidant activity by ABTS methods with IC50 ranging between, 25.5 and 35.84μg/ml. In addition, these new compounds were not toxic on the viability of macrophage cells J774A.1 at a concentration of 1 μM and the only non-toxic compound was P7.