EFFECT OF PILOCARPINE ON MINOR SALIVARY GLANDS OF RABBITS: A HISTOLOGICAL STUDY

Abstract

Back ground: Pilocarpine is a parasympathomimetic agent with mild β-adrenergic increase the rate of salivation. It is commonly used for treatment of patients with Sjögren's syndrome and post radiation salivary gland hypofunction. The intention of the present work was to describe the effects of Pilocarpine drug which is used as a drug with parasympathomimetic effect and the histological changes during treatment with it. Methods: Eighteen male rabbits were used in this experiment to show the effect of Pilocarpine . Different doses of drug 3mg/kg ,5mg/kg were given to treated groups A and B within the therapeutic limits. The experimental animals were injected intraperitoneally twice daily, for two and six weeks. Samples of the minor salivary glands were processed for light microscopy. Sections of the minor salivary glands were stained with H&E and PAS stains. Statistical analysis was followed to measure the diameter of the secretory acini. Results: The most noticeable changes were significant increase in the diameter of the secretory acini, and vacuolation with foamy appearance of the cells of the acini in treated groups. The PAS positive reaction in the acini was increased as the dose of the drug increased. Conclusion: Pilocarpine as sialogogues drug simulates the effect of parasympathomimetic drugs. The structural histological alterations noticed in this study substantiate the use of Pilocarpine as prophylactic and treated agents in patients suffering from xerostomia , patients receiving radiotherapy at head and neck malignancies, and some diseases like Sjögren's syndrome. Key words: Rabbits, minor salivary glands, histology, Pilocarpine, vacuolation.