Abstract:
The extracellular polymeric substances (EPS) extracted from Pseudomonas
putida
ATCC 11172, were used as model to quantify the effect of carbon concentration
on the chemical composition of EPS.P. putida was grown using Luria broth (LB)
medium with and without glucose in order to promote different EPS composition.
The influence of glucose amount was studied by supplementing the LB medium
with glucose at 0.5% w/v (LBG 0.5%) and 1.0% w/v (LBG 1%). The produced
EPS was characterized by Fourier transform infrared (FTIR) and X-ray
photoelectron spectroscopy (XPS). FTIR were used to describe the difference in
functional groups on free and bound EPS surfaces. XPS analysis was performed to
quantify the element surface composition, to evaluate the local chemical
environment of carbon and oxygen at the free and bound EPS, and to calculate the
overall concentration of polysaccharides, peptide and hydrocarbon compounds of
free and bound EPS. The combination of XPS and FTIR spectroscopy allows a
more comprehensive characterization of free and bound EPS surfaces.
