Effect of Naja haje (Egyptian cobra) crude venom- induced oxidative stress on the kidney of Wister albino rats

Abstract

In this study, oxidative stress inductions as well as nephrotoxicity of the Egyptian cobra crude venom at a doses of 0.025mg/kg (i.m.) of rats after 3, 6 and 9 h from envenomation. 90 rats divided into 3 groups, control group injected with 200 μl saline solution and group (2) injected with LD50 of cobra crude venom group (3) injected with1\2 LD50 of cobra crude venom . Quantitative evaluation of functional alterations in the kidneys were performed by biochemical analyses. Values of urea and creatinine significantly increased (P≤0.05) LD50 and ½LD50 at 3 h,6 h and 9 h. Oxidative stress biomarkers were assayed in serum levels of MDA was significantly at 3 h and nitrite 9 h while the levels of GSH, SOD and CAT were significantly decreased, especially after 9 h of envenomation. The levels of MDA and NO in serum were markedly increased especially at 3h and 9 h respectively, whereas The levels of serum GSH, SOD and CAT were decreased after 9 h respectively. These results suggest that, such effects which are so drastic may reflect probabilities of nephrotoxicity due to cobra envenomation with the involvement of oxidative stress as a potential mechanism